Leonurine inhibits breast cancer cell growth and angiogenesis via PI3K/AKT/mTOR pathway

Purpose: To elucidate the effect of leonurine on proliferation, invasion, migration, and angiogenetic potential breast cancer cells. Methods: Human cell line (MDA-MB-231) normal line, (SK-BR-3) were cultured. Both lines treated with 200, 400, or 800 μM cultured for 0 (control), 24, 48, 72 h. Cell counting kit-8 (CCK8) colony formation assays performed to measure viability proliferation. Invasion migration evaluated using in vitro invasion wound healing assays, respectively, while angiogenesis was by branching point structures. Furthermore, phosphorylation PI3K, AKT, mTOR assessed Western blot. viability, further investigated media including 740Y-P, leonurine, plus 740Y-P. Results: Leonurine inhibited proliferation cells weakened a dose-dependent manner (p < 0.05). repressed PI3K/AKT/mTOR pathway reducing mTOR. also progression Conclusion: inhibits inhibiting pathway, is thus, agent management cancer.